Differential expression analysis of a Vibrio parahaemolyticus ∆rpoN mutant compared to wild-type in minimal media supplemented with glucose or mouse intestinal mucus

Vibrio parahaemolyticus is the leading bacterial cause for seafood-related gastroenteritis worldwide. As an intestinal pathogen, V. parahaemolyticus competes with other commensal bacteria for the same pool of nutrients. The major source of nutrition for intestinal bacteria is intestinal mucus. We wanted to determine the expression profile of wild-type V. parahaemolyticus in mouse intestinal mucus and then perform a differential expression analysis in a ∆rpoN deletion mutant. RNA-Seq analysis was performed on RNA isolated from two biological replicates of wild-type Vibrio parahaemolyticus strain RIMD2210633 and two biological replicates of the ∆rpoN mutant strain, grown in M9 minimal media supplemented with glucose or mouse intestinal mucus.