RNA sequencing of control and GTD5(LOC_Os11g20384) knockout mutant in mature rice anthers (Oryza sativa japonica ssp.)

We generated single loss-of-function knockout mutant targeting a specific rice gene, GTrD5; LOC_Os11g20384), a member of the Suppressor of Actin 1 (Sac1) domain phosphoinositide phosphatase subfamily. GTrD5 is strongly expressed in mature anthers and pollen, and its knockout results in germination defects. Consequently, GTrD5 mutants have reduced self-fertilized seed production. This dataset comprises mature anther transcriptome data from GTrD5 single mutants, with samples collected from mature anthers for analysis.