Multi-omics characterization of human Embryonic Stem Cells-derived Pharyngeal Endoderm cells (RARα ChIP-Seq)

We have developed a novel in vitro protocol for the derivation of bona fide Pharyngeal Endoderm (PE) cells from hESCs. We demonstrated that our PE cells robustly express Pharyngeal Endoderm markers, they are transcriptionally similar to PE cells isolated from in vivo mouse development and represent a transcriptionally homogeneous population. Importantly, we elucidated the contribution of Retinoic Acid in promoting a transcriptional and epigenetic rewiring of PE cells. In addition, we defined the epigenetic landscape of PE cells by combining ATAC-Seq and ChIP-Seq of histone marks. We performed ChIP-Seq experiments for RARα on Anterior Foregut Endoderm (d5) and Pharyngeal Endoderm (d5 +RA) cells, and we identified the DNA regions bound by this transcription factor in the two cell types.