SNAT - Single cell RNA sequencing of mouse sorted Schwann cells at postnatal day 14 (P14)

Single cell RNA sequencing of sorted mouse Schwann cells expressing eYFP due to P0Cre-mediated recombination. Schwann cells were acutely extracted from P14 mouse pups which, besides of beta-actin dsred, also express eYFP upon P0cre-mediated recombination. The eYFP fluorescence allows the selection of eYFP positive Schwann cells from eYFP negative debris and non-Schwann cells in the nerve. 384-well plates were used to collect one cell per well, and these individual Schwann cells were further processed for library preparation using the SmartSeq2 chemistry. Two of these plates were sequenced at P14, each plate with cells derived from independent biological samples. The cells sorted into each plate derive from an original cell suspension containing pooled cells extracted from male and female mice in a 1:1 ratio.