Mouse WOI Stroke Data

Baseline imaging of two mice for five minutes on 11/18/22 ('201118') right before photothrombosis to left somatosensory cortex (2.2mm left and 0.5mm anterior to bregma with a 0.5mm spot size). Same imaging performed on same two mice for five minutes three days later 11/22/20 ('201122'). Each MATLAB file is 156 x 156 x 20160 representing pixels x pixels x frames. Each frame consists of an image of the dorsal cortical surface of each Thy1-GCaMP6f expressing mouse. Framerate of acquisition was 16.8Hz per LED channel. Four sequential LED channels were used; blue (470nm) for GCaMP6 excitation, green (530nm), yellow (590nm), and red (625nm) for hemoglobin spectroscopy. Data processing and analytic tools described in Brier and Culver (BioRxiv, April 2021).2022年11月18日（编号'201118'），在对左侧躯体感觉皮层实施光血栓形成术（相对于前囟点左2.2mm、前0.5mm，光斑尺寸0.5mm）之前，我们对两只小鼠开展了5分钟的基线成像。后续于三天后，即2020年11月22日（编号'201122'），对同两只小鼠进行了时长同为5分钟的相同成像操作。每个MATLAB文件的维度为156×156×20160，分别代表像素×像素×帧数。每帧图像均为表达Thy1-GCaMP6f的小鼠背侧皮层表面的成像结果。采集帧率为每个LED通道16.8Hz。实验共使用4个连续的LED通道：蓝光（470nm）用于GCaMP6激发，绿光（530nm）、黄光（590nm）及红光（625nm）用于血红蛋白光谱检测。本数据集的数据处理与分析工具详见Brier与Culver于2021年4月发布于BioRxiv的研究成果。