2D LSFM timelapse of cardiomyocyte calcium dynamics

Uploaded zip-folder contains the following files:<br> 1. A representative raw dataset of a 2D LSFM ventricular cardiomyocyte undergoing stimulated calcium transients and calcium sparks (frame_0000.tif -frame_17999.tif)<br> 2. The recorded pacing signal time trace (waveform_test.xslx)<br> 3. Image corresponding to the time-averaged background (AVG_19_35_39_LowNA rolling shutter.tif)<br> 4. Pre-processed nuclear mask matrix (NuclearMask.mat), CMO-channel average (CMO_Average.mat), and CMO channel maximum intensity projection (CMO_MIP). <br> 5. Split and co-registered data for each spectral channel (CMO_frame_00001.tif-CMO_frame_18000.tif, FLUO4_frame_00001.tif -FLUO4_frame_18000.tif).<br> <br> Compressed file size: 14.9 GB<br> Uncompressed file size: 42.8 GB. <br> <br> Related to the following manuscript: <br> Liuba Dvinskikh, Hugh Sparks, Ken MacLeod and Chris Dunsby " <em>High-speed 2D light-sheet fluorescence microscopy enables quantification of spatially varying calcium dynamics in ventricular cardiomyocytes</em>" (2023), <em>In review</em> with Frontiers in Physiology, Cardiac Electrophysiology.