Transcriptomic response of the thraustochytrid Aurantiochytrium to media richness and oxidative stress imposed by methylene blue

This study examines the transcriptomic responses of Aurantiochytrium limacinum to environmental stress, focusing on oxidative stress induced by methylene blue and the impact of nutrient availability. RNA-Seq was performed on both a wild-type strain and a carotenoid-deficient mutant (crtIBY knockout) to investigate the role of carotenoid biosynthesis in stress resistance. Additionally, gene expression profiles were compared between cultures grown in rich and poor media, highlighting the metabolic adaptations that occur under nutrient-limited conditions.