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Whole Exome sequencing of colorectal cancer patients (SG-BULK)

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NIAID Data Ecosystem2026-03-13 收录
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https://www.omicsdi.org/dataset/ega/EGAS00001006006
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The study was approved by the institutional review board of Singhealth (2018-2795). Ten 5-µm tissue sections were cut using standard microtomy techniques. Each collected cell population was then extracted using the allprep kit. Extracted material was then quantified using a Qubit fluorometer. DNA-seq libraries were captured to exome regions using xGen Exome Research Panel v1.0 (IDT), and libraries were prepared using the KAPA Hyper prep kit. DNA libraries were sequenced to a target depth of ×200 for tumor sample, ×100 for normal samples on the Illumina HiSeq platform.EGA study EGAS00001006006
创建时间:
2022-03-14
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