Topology of the redox network during induction of photosynthesis as revealed by time-resolved proteomics in tobacco

Employing a new time-resolved redox proteomic method, we have investigated the redox network in vivo during the dark-to-low-light transition. We show that redox states of some TRXs follow the photosynthetic linear electron transport rate. While some redox targets have kinetics compatible with an equilibrium with TRXf, reduction of other proteins shows specific kinetic limitations, allowing the fine-tuning of each redox-regulated step of chloroplast metabolism.