DANIO-CODE Prim-5 HiC

Zebrafish, a popular model for embryonic development and diseases, has so far lacked a systematic functional annotation programme akin to those in other animal models. To address this, we formed the international DANIO-CODE consortium and created the first central repository to store and process zebrafish functional genomic data. Our Data Coordination Center (https://danio-code.zfin.org) combines a total of 1802 sets of unpublished and reanalysed published genomics data, which we used to improve existing annotations and show its utility in experimental design. We identified over 140,000 cis-regulatory elements, including novel classes with distinct features dependent on their activity in time and space. We delineate the distinction between regulatory elements active during zygotic genome activation and those active during organogenesis, identifying new aspects of how they relate to each other. Finally, we match regulatory elements between zebrafish and mouse and predict functional relationships between them beyond sequence similarity, extending the utility of zebrafish developmental genomics to mammals.