Pathogenic IgE-fated memory B cell responses retain functional plasticity

To study the plasticity of IgE-fated allergen-specific responses, we employed various immunization methods to model type 1 and type 2 immune responses in mice, along with antibody (Ab)-mediated blockade of signaling receptors. Here, we treated epicutaneously sensitized mice with anti-IL-4Ra or an isotype control prior to s.c. reexposures and then performed paired single-cell RNA- and BCR-sequencing on B cells. An additional group of mice were administered cholera toxin and OVA to model a classical type 2 immunization strategy.