IRF2BP2 affects T-ALL cell proliferation by cooperating with master transcription factors [RNA-Seq]

Knocking down IRF2BP2 led to decreased T-cell acute lymphoblastic leukemia (T-ALL) cell survival and growth both in vitro and in vivo. To explore IRF2BP2-dependent gene regulation, RNA-seq analysis was conducted and the differently expressed genes were revealed in the IRF2BP2-knockdown J.gamma1 cells in comparison to control group. Overall design: mRNA profiles of J.gamma1 cells transfected with control shRNA-Control (shNC) or shRNA-IRF2BP2 (shIRF2BP2) were generated by RNA sequencing, in double, using Illumina NovaSeq6000. mRNA profiles of CD3+ T cells isolated from the spleens of Irf2bp2 fl-fl-/Vav-iCre+/- mice were generated by RNA sequencing.