RdRP-induced antiviral phenotype acts independently of intact adaptive immune signaling.

(A) RT-PCR analysis of Oasl2, Isg15, Ifit1, and Rig-I mRNA levels in cerebrum, spinal cord, and cerebellum of uninfected FVB mice (n = 5 per genotype, relative to Gapdh mRNA, mean ± SEM). (B) Cerebrum, spinal cord, and cerebellum tissue homogenates (n = 2 uninfected FVB mice per genotype, 1 per lane) were analyzed by immunoblotting using antibodies for RIG-I and ISG15. Antibody for β-ACTIN served as control. (C) RT-PCR analysis of viral titers in murine tissues two days post infection with EMCV (n = 8 mice per genotype, relative to endogenous Gapdh mRNA, mean ± SEM). (D) Survival curves (Kaplan-Meier plot) following EMCV infection (n = 8 mice per genotype). Data are representative of two independent experiments. *** P<0.001; **** P<0.0001.