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Environmental Nutrition Program (ENP) as an environmental intervention offered voluntary involvement with students but persists in exposure. This study aimed to investigate the effectiveness of ENP on students’ dietary intake and nutritional knowledge. With a quasi-experimental design, ENP comprised of four activities: the use of uniform food serving tools, nutrition fact displays, food and nutrition information display, and body weighing awareness. A multiple pass of 24-hour food recall and validated questionnaires were used in the assessments by nutritionist. Multiple linear regressions were performed analyzing the effectiveness of ENP. After 8-weeks, ENP activities are effective in increasing students’ daily carbohydrate intake as well as nutritional knowledge. ENP may be appropriate in enhancing nutrition literacy among female students.