Effect of the simulated shade on reproductive tissues (floral buds) of Arabidopsis thaliana

To investigate the effect of simulated shade on the genetic network regulating ovule development, we treated Arabidopsis adult plants with W or simulated shade (W+FR) for up to 24 h. After extracting total RNA from floral buds we performed gene expression profiling analysis using data from RNA-seq of two different treatments at different time points Overall design: Wild-type (Col-0) and mutant (MB2 line, an inducible transgenic line expressing pAP1:AP1-GR in an ap1 cal background) Arabidopsis plants were grown under LD conditions (W; high R:FR) until they started to flower. Then, plants were treated with simulated shade (W enriched with FR, W+FR; low R:FR) and floral buds were harvested at 0, 1, 3 and 24h. Samples were also harvested from control plants grown for 24 h under W. In the case of MB2 line, inflorescences were induced treating the plants with 2 µM dexamethasone 3 days before the simulated shade treatment