Jennifer Bestman, Holly Cline (2011) CIL:37063, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #3 (time point: 2 hours)of an image group. It shows a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

In the series, z-stacks of this neuron were acquired at a 2 hour interval for 8 hours. Afterward, a second time lapse series was acquired at a 60 second interval for 10 minutes. This time series shows dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为图像组中的第3幅图像（时间点：2小时）。图中呈现了无色斑马鱼（Xenopus laevis）中枢神经系统视网膜的神经元，处于发育的第47阶段。在该系列图像中，以2小时为间隔，对这一神经元的z轴切片进行了持续8小时的采集。随后，在60秒的间隔下，又进行了为期10分钟的第二次时间序列采集。该时间序列图像展示了树突枝干的发育和形态可塑性，包括枝干的增生与退缩。在图像采集前约24小时，细胞通过体内电穿孔法转染了编码增强型绿色荧光蛋白（eGFP）的质粒DNA。整个活体蝌蚪在麻醉状态下，被放置在玻片下，并通过定制构建的双光子显微镜直接穿过其透明的皮肤进行成像。