Transcriptome Profile of C. Pseudotuberculosis in Response to Iron Limitation: Differential gene expression of Iron and DtxR regulated genes

RNA-seq differential gene expression profiling of Corynebacterium pseudotuberculosis under iron limitation. We use two C. pseudotuberculosis strains, a ciuA Cp13 mutant and its parental wild type T1 strain to quantify the relative gene expression of these strains in cultures with low iron availability. The ciuA Cp13 mutant is deficient for an operon which encodes a iron siderophore uptake system. The Cp13 mutant also showed reduced virulence in CLA infection models. Due to the importance of iron for many pathogenic bacteria, our primary goal was to identified the expression profile of iron regulated genes. Overall design: Differential gene expression of two strains of C. pseudotuberculosis under iron limitation