Investigation of the transcriptional profile of stimulated HUMAN CRISPR/Cas9 MOCK and A2ARKO α-lewisY CAR T cells in the presence/absence of NECA

Investigation of the transcriptional profile of sorted and stimulated CD8 and CD4 murine CRISPR/Cas9 MOCK and A2ARKO α-her2 CAR T cells in the presence/absence of NECA Following CRISPR/Cas9 mediated editing of T cells with either an A2AR sgRNA or a non-targeting control (MOCK), cells were transduced to express the anti-Her2 CAR. After 7 days culture in IL-2 (100 U/ml) and IL-7 (200 pg/ml), MOCK or A2AKO CAR T cells were stimulated with plate bound anti-myc Tag (anti-CAR) antibody in the presence or absence of NECA (1 µM) for 5 hours. CAR T cells were then sorted for CD8 and CD4 subsets by flow cytometry before being analyzed with 3' RNA-seq.