Phosphorylated-Ser807 STAT6 protects macrophages from senescence

STAT6 play a key role in homeostasis of macrophages. We identified a new modification of STAT6 at Ser807, which may contribute to STAT6 function. Thus, we generated STAT6 point mutant mice (S807A and S807E). We sequenced the mRNA of macrophages from these mutant mice to rule out the function of the specific phosphorylation. Overall design: Primary Thio-elicited peritoneal macrophages were isolated from WT and mutant mice. After short-term adherent, the PMs were collected for RNA extraction.