Proteomic Analysis of Extracellular Vesicles Derived from Human Pluripotent Stem Cells

Extracellular vesicles (EVs) and secretory factors are key to intercellular communication, yet their molecular dynamics in human pluripotent stem cells (hPSCs) are not well understood. We identified a key EV surface protein that regulates hPSC stemness, enhances growth, and promotes EV endocytosis. EV internalization helps reduce oxidative stress and cell death by transporting redox and stress response proteins, highlighting the importance of EV surface proteins in hPSC self-renewal and survival.