Spatiotemporal Maps of DNA Methylation Across the Chromatin Accessible Genome in a Monocyte to Macrophage Transition

In this study a coupled methodology of assay for transposase accessible chromatin (ATAC) and bisulfite conversion was applied to stimulated monocytes. This coupled methodology yielded tandem insights into DNA methylation and chromatin accessibility at activating gene regulatory elements. Paired with transcriptomic data this study identified static DNA methylation at genomic regions highly dynamic for chromatin accessibility yet robust gene expression changes in response to monocyte stimulation.