Effect of pulpal hydrostatic pressure on the morphological data of the fluid droplets emerging from dental enamel in human teeth

In vivo replicas were obtained in the mid buccal premolars using polyvinylsiloxane impression material. Positive replicas were then collected using a polyether impression material. All replicas were inspected by a scanning electron microscope (SEM).
In vitro, each tooth was prepared for fluid flow measurement during which the pulp chamber was filled with Ringer’s solution and the pressure in the pulp cavity was set at 0, 20, 100 and 200 mmHg above atmospheric. All replicas were obtained with each of the different pressures exactly the same procedure as that of in vivo. This study was supported by the Thailand Research Fund (RSA 6180038).