Differential gene expression in Hep3B and Hep3B SOX4-/- cells. Differential gene expression in Hep3B and Hep3B SOX4-/- cells

Overexpression of SOX4 in various kinds of cancers specimen was associated with poor prognosis of patients; however, the role of SOX4 in angiogenesis or tumor microenvironment modulation remains unclear. Therefore the endogenous SOX4 was knockout and the differential gene expression between Hep3B and Hep3B SOX4-/- cells were examined via genechip. We found that the differentially expressed genes, EzH2, a SOX4-associated partner, and CXCL12, were repressed in Hep3B SOX4-/- cells compared with parental Hep3B; these results were further assessed via qRT-PCR in Hep3B SOX4-/- versus Hep3B cells. Overall design: Endogenous SOX4 knockout in Hep3B cells via the CRISPR/cas9 system that was designed as Hep3B SOX4-/-, the transcriptome of Hep3B and Hep3B SOX4-/- cells were analyzed via an Affymetrix GeneChip™ Human Transcriptome Array 2.0.