Description of pulsed light conditions employed for Nannochloropsis salina growth.

Alternating cycles of light and dark were all designed to have same integrated light intensity (Ia), corresponding to 120 µE m−2 s−1 of continuous light. Flashes of two intensities were employed, 1200 and 350 µE m−2 s−1, with duty cycles of 0.1 and 0.33. Light changes made at different frequencies, 10, 5, 1 Hz with 1200 µE m−2 s−1 and 10, 30 Hz with 350 µE m−2 s−1. Pulsed light conditions resulted in precise duration of flashes (tf) and dark (td). See also Figure S2.