Oryza sativa Raw sequence reads. Oryza sativa

As a means to understand the mechanistic underpinnings of biolistic transformation, we transformed linear and circular DNA molecules into rice and maize, and subjected the lines to whole genome sequencing and analysis. The study of the transgene breakage and genome damage revealed a wide spectrum of insertions and outcomes, from simple insertions to long shattered arrays. Multiple forms of genome damage were observed, including chromosome breakage and shattering and extreme copy number variation.