Figure 2D - EPR10411 + 11A9 Raw Image Files

Raw image files to:<br>Figure 2. Further analysis of Ago2 localization to the iRBC. (A) Binding sites of aAgo2 antibodies used. The monoclonal antibodies 11A9 and EPR10411 were raised against an N-terminal and a centrally located peptide epitope, respectively. The antibody 2E12-1C9 is a monoclonal antibody raised against the whole C-terminus of hAgo2. (B) IFA of uninfected RBCs. Yellow: Ago2 (EPR10411), magenta: Ago2 (either 2E12-1C9 (top) or 11A9 (bottom)). (C) IFA of P. falciparum 2004 parasites at 10, 22, 34 and 46 hours post invasion (hpi) and mature PfNF54 gametocytes. Yellow: Ago2 (EPR10411), magenta: Ago2 (2E12-1C9). Contrast and brightness of EPR10411 panels were individually adjusted to optimise image quality, thus, signal strength cannot be compared between panels. (D) IFA of P. falciparum 2004 parasites at 10 and 46 hpi. Yellow: Ago2 (EPR10411), magenta: Ago2 (11A9). Nuclei were stained with Hoechst (blue). Images were taken on a Leica widefield microscope (60x objective). Representative images of at least 5 per condition are shown. Controls using only a secondary antibody revealed no unspecific staining. Scale bar indicates 5 µm.