BAT Hi-C maps global chromatin interactions in an efficient and economical way

We describe the Bridge linker-Alul-Tn5 Hi-C (BAT Hi-C) method, which is a simple and efficient method for delineating chromatin conformational features of mouse embryonic stem (mES) cells and uncover DNA loops. This protocol combines Alul fragmentation and biotinylated linker-mediated proximity ligation to obtain kilobase (kb) resolution with a marked increase in the amount of unique read pairs. The protocol also includes chromatin isolation to reduce background noise and Tn5 tagmentation to cut down on preparation time. BAT Hi-C data in Untreated mES cells. Reference: The SRA Study accession (SRP218781) and BioProject accession (PRJNA561038)