Hypoxia disrupts metabolism in coral and sea anemone larvae

Anthropogenic pollution is driving an increase in the frequency and severity of seawater hypoxic events in coastal marine ecosystems. Although hypoxia decreases physiological performance in coral and sea anemone (phylum Cnidaria) larvae, the underlying cellular mechanisms remain unexplored. Here, larvae of the reef-building corals Galaxea fascicularis and Porites astreoides and the estuarine sea anemone Nematostella vectensis were exposed to normoxia or a simulated hypoxic event (6 h at <2 mg dissolved O2 l−1), and their metabolomic response was quantified at the end of the exposure period using targeted liquid chromatography-mass spectrometry. Baseline metabolite profiles (81 amino acids, acylcarnitines, organic acids and nucleotides) were broadly divergent between the three species, with the corals displaying a reliance on nitrogen cycling through amino acid metabolism, whereas N. vectensis relied on nucleotide metabolism. By contrast, s..., Groups of larvae were exposed to normoxic or hypoxic conditions for 6 h, flash-frozen, and then processed for LC-MS metabolomics at the University of Pennsylvania Metabolomics Core. Further details can be found in the associated Open Access publication at: https://doi.org/10.1242/jeb.250372., , # Data from: Hypoxia disrupts metabolism in coral and sea anemone larvae

This dataset contains targeted LC-MS metabolomics data for coral and sea anemone larvae exposed to normoxic and hypoxic conditions. Further information can be found in the associated Open Access publication at: [https://doi.org/10.1242/jeb.250372](https://doi.org/10.1242/jeb.250372).

## Data and file structure

### File: Metabolomics_data.csv

This file contains sample metadata and abundance data for all metabolites quantified at the University of Pennsylvania Metabolomics Core.

These files have headers, which include:

* Species = the species from which metabolomics data originate
* Treatment = experimental treatment (normoxia or hypoxia)
* Cohort = the spawning cohort from which larvae originate (not relevant for this dataset)
* Group = replicate group within the experimental treatment (1-3)
* Life_stage = always \"larva\"
* Hours_post_treatment = time point at which larvae were collected for metabolomics; here,...,