Cloning of White Rust Resistance gene WRR12 from Arabidopsis thaliana

Arabidopsis lines MAGIC.329 and MAGIC.23 are resistant or partially resistant respectively to B. oleracea-infecting A. candida race AcBoT. To identify the underlying WRR genes, MAGIC.329 x MAGIC.23 lines were crossed and F2 progeny was obtained. Approximately 200 fully susceptible F2 individuals following AcBoT inoculation were collected and bulked (Bulk Susceptible) for DNA extraction. Resistance gene Enrichment Sequencing (RenSeq) experiments were conducted on DNA of the parents (MAGIC.329 and MAGIC.23) as well as bulk susceptible DNA (BS). Illumina MiSeq Sequencing technology was then used on enriched DNA samples.