Confocal images of protocerebro calycal tract neurons colocalized with GABA and GAD immunostaining in Apis mellifera

Confocal images (Leica TCS SP2), scan mode xyz, format 1024x1024, beam expander 6, pinhole 1, 40 x oil immersion objective (400 times magnification, plus digital zoom in some cases, see leica info files (txt)) of the mushroom body of the bee brain, including somata (So), pedunculus (Ped), calyx (Cal) and alpha lobe (aLob). Immunostaining was against GABA and GAD and Neurobiotin was used for intracellular staining. Filenames include the series index (n) and the date (associated number).