Development of Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction for the quantification of five steroid hormones: supplementary materials

Aim: An accurate and sensitive analytical method was proposed to detect some steroid hormones
in biological samples. Materials & methods: An Fe3O4/reduced graphene oxide nanocomposite-based
dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid
hormones from human serum samples. Results & conclusion: The nanocomposite was firstly used as
adsorbent to simultaneously extract the selected hormones. Limit of detection values for the selected
hormones were calculated between 5.5 and 39.2 ng/kg (mass based). An artificial serum sample was used
to test the applicability and accuracy of the developedmethod; percentage recovery results obtained from
two different spiked concentrations were found to be in the range of 80.5–99.9%.

目标：提出了一种准确且灵敏的解析方法，旨在检测生物样本中的某些类固醇激素。材料与方法：开发了一种基于Fe3O4/还原氧化石墨烯纳米复合材料的分散固相萃取技术，以实现从人血清样本中高效且简便地富集类固醇激素。结果与结论：该纳米复合材料首先作为吸附剂，用于同时提取选定的激素。所选激素的检测限值在5.5至39.2 ng/kg（质量基准）之间。使用人工血清样本测试了所开发方法的适用性和准确性；从两种不同的加标浓度获得的回收率结果在80.5%至99.9%之间。