Total data_BA

Methods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic beads (Precellys). For each 50 mg of tissue, 300 μl of methanol and the deuterated internal standards were added and tissues were homogenized in a Precellys 24 Dual system equipped with a Criolys cooler (Precellys). Samples were analyzed using an Acquity UPLC system (Waters, UK) equipped with an Acquity UPLC BEH C18 column (1.7μm, 2.1 x 100 mm; Waters). The MS analysis was performed using a Waters Xevo TQ-XS mass spectrometer (Waters) with an ESI source working in the negative-ion mode.