Transcriptome profiling of NCI-H2030 cell line treated with adagrasib, K-975 and adagrasib+K-975

NCI-H2030 lines were seeded in 6-well plates in complete culture medium and cultured at densities allowing exponential growth: 50000 cells/well. After 24 h, compounds were added (DMOS, adagrasib (30 nM), K-975 (100 nM) and adagrasib (30 nM)+K-975 (100 nM)) at a final percentage of 0.2% DMSO. Twenty-four hours after compounds addition, cells were used for transcriptomic profiling. Overall design: Transcriptomic proofing of DMOS, adagrasib (30 nM), K-975 (100 nM) and adagrasib (30 nM) + K-975 (100nM) with two technical and three biological replicates (4 conditions X 2 technical replicates X 3 biological replicates =24 samples).