Data archive for: Resting cells of Skeletonema marinoi assimilate organic compounds and respire by dissimilatory nitrate reduction to ammonium in dark, anoxic conditions

Data archive for: “Resting cells of Skeletonema marinoi assimilate organic compounds and respire by dissimilatory nitrate reduction to ammonium in dark, anoxic conditions” https://doi.org/10.1111/1462-2920.16625

Dataset of single cell assimilation of organic/inorganic C/N by resting cells of the marine diatom Skeletonema marinoi captured using secondary ion mass spectrometry (SIMS) and stable isotopic tracers. The dataset also contains POC/PON changes over time during dormancy, DNRA (15N-NH4+ production), denitrification (15N-N2 production) and a germination assay to determine survival rate, most probable number analysis (MPN).  

Two strains (GF04 and R05) were incubated in dark and anoxic conditions in two different incubation experiments.

Incubation 1: Diatoms treated with antibiotics before entering dormancy compared to a control not treated with antibiotics then given 15N-NO3- in dark anoxic conditions.

Incubation 2: Diatoms treated with antibiotics given, 15N & 13C urea, 15N & 13C urea + 14N-NO3-, 13C-acetate, 13C-acetate + 15N-NO3-, or 15N-NO3-.

See the main manuscript for a extensive experimental setup.

Each file is uploaded as both a .CSV and .XLSX, so that you can choose which you prefer.

DNRA_and_denitrification.csv/xlsx: DRNA and denitrification depending on volume (Incubation 1)

DNRA_per_cell.csv/xlsx: DNRA per cell (Incubation 1 & 2)

MPN_data.csv/xlsx: Most probable number analysis (Incubation 1 & 2)

POC_PON.csv/xlsx: POC and PON per cell and volume (Incubation 1 & 2)

SIMS_data.csv/xlsx: SIMS data (Incubation 1 & 2)