Inhaled multi-walled carbon nanotubes-induced gene expression profile in rat lung (NM-401)

In this work we study the pulmonary toxicological properties of carbon nanotubes using molecular toxicological approache. For this, we exposed Sprague Dawley rats by nose-only inhalation 6 hours/day, 5 days/week for 4 weeks. Lung samples have been collected up to 180 days after the end of exposure and transcriptomics analysis were performed. 13 week old female Sprague Dawley rats were exposed by nose-only inhalation 6 hours/day, 5 days/week for 4 weeks to two doses of carbone nanotubes (NM-401). Carbon nanotube-induced gene expression profile in rat lung was performed 3, 30, 90 and 180 days post exposure (4 time points). Each time point is compsed of 6 control, 6 exposed rats to a low dose and 6 exposed rats to a high dose. A total of 72 samples (microarrays) was used.