Chromobox homolog 4 overexpression inhibits TNF-a-induced matrix catabolism and senescence by suppressing activation of the NF-?B signaling pathway in nucleus pulposus cells

Chromobox homolog protein 4 (CBX4) exhibits anti-inflammatory effects and shows promising therapeutic potential. Thus, in the present study, we explore the role of CBX4 in IDD. Overall design: For RNA sequencing, total RNA was extracted using Trizol from 4 groups of rat NP cells, i.e., control cells, CBX4-overexpressing cells, TNF-a-stimulated cells, and CBX4-overexpressing cells stimulated with TNF-a.