Transcriptomic analysis of amp1-13 and cyp78a5,7 Arabidopsis seedlings

ALTERED MERISTEM PROGRAM1 (AMP1) is a member of the M28 family of carboxypeptidases with a pivotal role in cell fate maintenance in the embryo and shoot meristem. A defect in AMP1 function results in suspensor to embryo conversion and a hypertrophic shoot meristem forming ectopic stem cell pools. However, so far the role of AMP1 in shoot development could not be assigned to a specific molecular pathway nor is its biochemical function resolved. Double mutants in CYP78A5 and CYP78A7 develop a similar set of cell fate defects. To further assess whether this phenotypic overlap is also depicted in a congruency at the global gene expression level, we analyzed the transcriptomic responses of both genotypes Seedlings of indicated genotypes were germinated and grown for 10 d in liquid medium. For each genotype three biological replicates were used for RNA extraction and subsequent hybridization on Affymetrix microarrays.