ACLY Cut&RUN on liver
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https://zenodo.org/record/10497640
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For CUT&RUN, we utilised the EpiCypher inc CUTANA ChIC/CUT&RUN kit (EpiCypher, SKU: 14-1048) according to the manufacturer’s instructions with modifications. We used 6x104 cells per sample from the nuclear isolation buffer (NIB, 15 mM Tris-HCl (Carl Roth, Art.-Nr. 9090.3) pH 7.5, 60 mM KCl (Carl Roth, Art.-Nr. 6781.3), 11 mM CaCl2 (Carl Roth, Art.-Nr. CN93.1), 5 mM NaCl (Carl Roth, Art.-Nr. 3957.2), 5 mM MgCl2 (Carl Roth, Art.-Nr. KK36.2), 250 mM sucrose (Sigma, S0389), 1 mM 1,4-Dithiothreitol (Carl Roth, Art.-Nr. 6908.3), 10 mM sodium butyrate (Sigma Aldrich, 303410)) supplemented with 0.3% (v/v) IGEPAL CA-630 (Sigma, I8896) and 1X cOmplete EDTA-free protease inhibitor cocktail (Roche, 11836145001).
创建时间：
2024-01-19
