Expression data from human alveolar macrophages (AMs) of fetal versus adult origin

The impact of cell origin on human lung macrophage identity and function remains unknown. In this study we characterized human alveolar macrophages of fetal versus adult origin. We used microarray to define the gene signatures of human alveolar macrophages derived from CD116+CD64+ fetal monocytes, CD116+CD64- fetal precursors, and CD34+ HSPCs in MISTRG humanized mice. Human cells with an alveolar macrophage surface phenotype (CD45+CD11b+HLA-DR+CD206+CD169+) were purified from MISTRG mice 13 weeks after intrahepatic transplantation of newborn mice with either adult (2 x 10^5 CD34+ HSPCs) or fetal precursors (2 x 10^5 CD34-Lin-CD116+CD64- or CD34-Lin-CD116+CD64+ fetal liver cells). HSPC-engrafted MISTRG mice were injected intravenously with 2 μg of PE-conjugated anti-human CD45 antibody (HI30, BioLegend) before lung harvest to exclude IV CD45-PE+ pulmonary intravascular macrophages from the sorted CD45+CD11b+HLA-DR+CD206+CD169+ subset as described (Evren et al., 2021)