HC2091 kills Mycobacterium tuberculosis by targeting the MmpL3 mycolic acid transporter

The purpose of this study was to characterize the mechanism of action of the small molecule HC2091 Mtb strain CDC1551 was grown at 37ºC in T-25 vented, standing tissue culture flasks in 8 mL of buffered 7H9 medium to an OD600 of 0.1. Cultures were subsequently treated with 10 µM HC2091 ro SQ109. For the control, cultures treated with an equal amount of DMSO. Following a 24 h incubation period, total bacterial RNA was extracted, quality controlled and sequenced. Each treatment was repeated with two independent (biological) replicates.