Lake Sturgeon Thermal Physiology RNA-seq from Burntwood and Winnipeg River populations, March 8 '22

Lake sturgeon RNA-seq from Burntwood River and Winnipeg River populations of lake sturgeon within Manitoba, CA. Prior to sequencing, mRNA from gill samples was extracted using a PureLink RNA Mini Kit, checking for RNA purity, concentration, and integrity using a NanoDrop One and gel electrophoresis. Next, samples were run on a Bioanalyzer to confirm RNA quality, and then mRNA isolation was performed using NEBNext Poly(A) Magnetic Isolation Modules (New England Biolabs) followed by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) to produce stranded cDNA libraries. NEBNext dual adaptors (New England Biolabs) were then individually applied to barcode each library prior to sequencing of 100 base pair paired-end reads. All 36 sturgeon gill samples were then sequenced on a single NovaSeq 6000 (Illumina) lane.