Dataset periodontitis y2021m02

<b>Description and susceptibility of periodontopathic bacteria with severe chronic periodontitis among a group of Peruvian patients: A pilot study</b> <b> </b> <b>ABSTRACT</b> <b>Background</b>: Severe periodontal disease is highly prevalent worldwide, affecting 20 % of the population between the ages of 35 and 44 years old. The etiological epidemiology in Peru is scarce, even though some studies describe a prevalence of 48.5 % of periodontal disease in the general population. Periodontitis is one of the most prevalent oral diseases associated with site-specific changes in the oral microbiota and it has been associated to socioeconomic state. This study aimed to determine the etiology and resistance profile of bacteria identified in a group of patients with chronic severe periodontitis. <b>Methods</b>: Six subgingival plaque samples were collected from eight patients with chronic severe periodontitis. Bacterial identification was carried out by an initial culture, PCR amplification and subsequently DNA sequencing. We evaluated the antibiotic susceptibility by disk diffusion method. <b>Results</b>: Variable diversity in oral microbiota was identified in each one the eight patients. The bacterial genus most frequently found was <i>Streptococcus </i>spp. (15/48, 31.3 %) followed by <i>Rothia </i>spp. (11/48, 22.9 %), <i>Actinomyces</i> spp. (9/48, 18.8 %), and <i>Eikenella</i> spp. (4/48, 8.3 %). The most common species found was <i>Rothia dentocariosa</i> (8/48, 16.7 %). The antimicrobial susceptibility assay varied according to the species tested, however, among all the isolates evaluated <i>Actinomyces naeslundii</i> was resistant to penicillin and tetracycline; <i>Eikenella corrodens</i> resistant to dicloxacillin; and <i>Rothia dentocariosa</i> was resistant to amoxicillin + clavulanic acid and metronidazole but also susceptible to trimethoprim-sulfamethoxazole. <b>Conclusions</b>: Bacterial species of importance medical were detected in patients with chronic severe periodontitis, especially <i>Rothia dentocariosa</i>, most prevalent in this study. Specific antimicrobial therapy is required to improve the treatment outcomes of patients with periodontal disease and to avoid the development of antibiotic resistance.

秘鲁患者群体中重度慢性牙周炎致病细菌的易感性分析：一项预实验研究

## 摘要
### 研究背景
重度牙周疾病在全球范围内流行率极高，影响20%的35至44岁人群。秘鲁地区的病因流行病学研究较为匮乏，尽管已有研究显示该国普通人群牙周疾病患病率达48.5%。牙周炎是最为普遍的口腔疾病之一，与口腔微生物群的位点特异性改变密切相关，且与社会经济状况存在关联。本研究旨在明确一组重度慢性牙周炎患者体内分离细菌的病因学特征及其耐药谱。

### 研究方法
从8名重度慢性牙周炎患者体内采集6份龈下菌斑样本。首先通过细菌培养、聚合酶链式反应（PCR）扩增及后续DNA测序完成细菌鉴定。采用纸片扩散法评估抗生素敏感性。

### 研究结果
8名患者的口腔微生物群多样性存在个体差异。检出率最高的细菌属为链球菌属（*Streptococcus* spp.，15/48，31.3%），其次为罗氏菌属（*Rothia* spp.，11/48，22.9%）、放线菌属（*Actinomyces* spp.，9/48，18.8%）以及侵蚀艾肯菌属（*Eikenella* spp.，4/48，8.3%）。最常见的检出菌种为龋齿罗氏菌（*Rothia dentocariosa*，8/48，16.7%）。抗菌药物敏感性检测结果因菌种而异：在所评估的所有分离株中，内氏放线菌（*Actinomyces naeslundii*）对青霉素与四环素耐药；侵蚀艾肯菌（*Eikenella corrodens*）对双氯西林耐药；龋齿罗氏菌对阿莫西林+克拉维酸及甲硝唑耐药，但对甲氧苄啶-磺胺甲恶唑敏感。

### 研究结论
本研究在重度慢性牙周炎患者体内检出了具有临床重要性的细菌菌种，其中以本次研究中检出率最高的龋齿罗氏菌尤为突出。需采用针对性抗菌治疗以改善牙周疾病患者的治疗效果，避免抗生素耐药性的产生。