Expression changes in Tetrahymena thermophila TRX1 and EZL2 gene knockouts and after hydroxyurea (HU) treatment. Tetrahymena thermophila

12 genome-wide microarrays to investigate the Tetrahymena thermophila gene expression changes in TRX1 and EZL2 gene knockout and hydroxyurea (HU) treatment conditions. Overall design: For the TRR1 knockout (KO) condition, wild type (WT427) cells and TRX1 KO cells were cultured using 1XSPP, 30 degrees, 100 rpm shaking, until 350,000 cells/ml. For the EZL21 knockout (KO) condition, wild type (WT427) cells and EZL2 KO cells were cultured using 1XSPP, 30 degrees, 100 rpm shaking, until 350,000 cells/ml. For the HU treatment condition, HU was used to treat the WT427 cells for 48 hours, setting wild type (WT427) as the control; cells were cultured using 1XSPP, 30 degrees, 100 rpm shaking. Each sample has two biological replicates.