16S microbiome profiling of maternal rats under methionine-deficient diet

This project aims to investigate how maternal methionine deficiency (MD) alters the gut microbiota composition in rats. To understand the role of the microbiome in mediating MD-induced metabolic changes, we performed 16S rRNA gene sequencing of maternal cecal contents. The analysis revealed compositional shifts in the gut microbiota, notably an increased abundance of Ruminococcus, which may play a role in regulating maternal-fetal metabolic signaling.