Evaluation of transcriptional changes following stimulation of naive, TCM, TEM and TEMRA CD8+ T cell subsets with AB248 compared to IL-2

Evaluation of transcriptional changes following stimulation of naive, TCM, TEM and TEMRA CD8+ T cell subsets confirmed that AB248 recapitulated the expected IL-2 signature with high fidelity. Human PBMCs from 3 donors were sorted into the following subsets based on the expression of CD45RO and CD62L markers: CD8+ naive T cells (CD62L+CD45RO-), CD8+ central memory T cells (CD62L+CD45RO+), CD8+ effector memory T cells (CD62L-CD45RO+), and CD8+ effector cells (CD62L-CD45RO-). Briefly, cells were stained with antibodies to sort the individual CD8+ T cell subsets, sorted via FACS, and treated at concentrations corresponding to the EC99 by pSTAT5 of rhIL-2 or AB248 for 24 h. At the end of stimulation, cells were pelleted, and RNA isolated per manufacturer's instructions and sent for sequencing using Ultra-Low Input RNA-Seq protocol (detects greater than 10 pg RNA).