Super-Resolution Monitoring of Mitochondrial Dynamics upon Time-Gated Photo-Triggered Release of Nitric Oxide

Nitric
oxide (NO) potentially plays a regulatory role in mitochondrial
fusion and fission, which are vital to cell survival and implicated
in health, disease, and aging. Molecular tools facilitating the study
of the relationship between NO and mitochondrial dynamics are in need.
We have recently developed a novel NO donor (NOD550).
Upon photoactivation, NOD550 decomposes to release two
NO molecules and a fluorophore. The NO release could be spatially
mapped with subdiffraction resolution and with a temporal resolution
of 10 s. Due to the preferential localization of NOD550 at mitochondria, morphology and dynamics of mitochondria could be
monitored upon NO release from NOD550.