Systematic analysis to analyze the Drs2-I(S/R)TTK motif: comparative analysis bertween the interactome of Drs2 vs. drs2-5A by CS-MS.

The aim of the project was to systematically analyze the mechanism mediated by the Drs2 ISTTK motif in yeast, located in the N-terminal tail of the protein. Upon a GFP-specific pulldown, we carried-out a comparative analysis between the interactome of Drs2-GFP and drs2-5A-GFP (ISTTK>AAAAA) to identify proteins whose binding required to recognize directly, or in the near vicinity, the ISTTK motif of Drs2.