RNA-seq of E0771-Vector control, E0771-Malt1-WT and E0771 Malt1-PD (Without T cell coculture)

To reveal the downstream paracrine signals from WT-Matl1 or PD-Malt1 tumor cells which responsible for the above observed macrophage phenotypes, we collected mRNA samples from Vector control-, WT-Malt1-, PD-Malt1-, V87R-Malt1-, or L88D-Malt1-expressing E0771 cancer cells, and performed RNA-seq analysis. E0771 cells expressing either -Vector control, wild type-Malt1 (M-WT), Malt1 PD mutant (M-PD), Malt1 V87R and Malt1 L88D were lysed by TRIZOL for RNA extraction, perform reverse transcription and cDNA library for RNA-Seq analysis.