Single-Cell Transcriptomics of Genome-Edited Kidney Organoids

We report the first use of genome-edited human kidney organoids, combined with single-cell transcriptomics, to study APOL1 risk variants at the native genomic locus in different nephron cell types. This approach captures interferon-mediated induction of APOL1 gene expression and cellular dedifferentiation with a secondary insult“second hit” of endoplasmic reticulum stress. Examination of interferon gamma +/- tunicamycin in G0 and G1 APOL1 kidney organoids. A, B, C, etc refer to independent experimental replicates.