Fold increase in gene expression in chickens after S. Enteritidis infection determined by real-time PCR.

Inf – upregulation in the naive infected chickens, Vacc – upregulation in the vaccinated and infected chickens. Upregulation in gene expression in the spleen was determined 4 days after intravenous infection of 42-day-old chickens, upregulation in the cecum was determined 3 or 4 days after oral infection of 1- or 42-day-old chickens, respectively. Background in red color, significant upregulation when compared with the expression in appropriate non-infected controls. Data in bold, significant difference between the expression in the spleen of infected and vaccinated and infected chickens.